Plasmodium vivax: Reverse transcriptase real-time PCR for gametocyte detection and quantitation in clinical samples
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چکیده
منابع مشابه
Comparison of real - time reverse transcriptase ( RT ) - PCR assays for 1 detection of swine hepatitis E virus in fecal samples
5 a Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 6 Medicine, Iowa State University, Ames, Iowa, USA 7 b Laboratório de Pesquisa em Virologia Animal, Departamento de Medicina Veterinária 8 Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Brazil 9 Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine ...
متن کاملComparison of real-time reverse transcriptase PCR assays for detection of swine hepatitis E virus in fecal samples.
Hepatitis E virus (HEV) is a major cause of acute viral hepatitis in people in many developing countries and is also endemic in many industrialized countries. Mammalian HEV (mHEV) isolates can be divided into at least four recognized major genotypes. Several nucleic acid amplification techniques have been developed for mHEV detection, with great differences in sensitivity. The aim of this study...
متن کاملA sensitive and quantitative single-tube real-time reverse transcriptase-PCR for detection of enteroviral RNA.
BACKGROUND Enteroviruses (EVs) are significant human pathogens. Rapid and sensitive diagnostic techniques are desirable. OBJECTIVES To develop a quantitative single-tube real-time reverse transcription-polymerase chain reaction (RT-PCR) for human enterovirus ribonucleic acid (RNA) (QPCR), with protection against amplimer contamination. STUDY DESIGN The method was evaluated with serial dilut...
متن کاملDetection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay
Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...
متن کاملComparison of FilmArray and Quantitative Real-Time Reverse Transcriptase PCR for Detection of Zaire Ebolavirus from Contrived and Clinical Specimens.
Rapid, reliable, and easy-to-use diagnostic assays for detection of Zaire ebolavirus (ZEBOV) are urgently needed. The goal of this study was to examine the agreement among emergency use authorization (EUA) tests for the detection of ZEBOV nucleic acids, including the BioFire FilmArray BioThreat (BT) panel, the FilmArray BT-E panel, and the NP2 and VP40 quantitative real-time reverse transcripta...
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ژورنال
عنوان ژورنال: Experimental Parasitology
سال: 2012
ISSN: 0014-4894
DOI: 10.1016/j.exppara.2012.08.010